Vitamin K for Chickens

By Nick
Medically Reviewed by Dr. A. Ibrar, DVM, Poultry Nutritionist
University of Veterinary and Animal Sciences, IUB, Selçuk Üniversitesi

Vitamin K is an essential fat-soluble vitamin for chickens. It regulates blood clotting and bone health. Optimal values will improve egg laying and growth.

But what are the optimal values? When should we be afraid of deficiencies, and how can we recognize them? And why should you care?

  • DURVET POULTRY VITAMINS & ELECTROLYTES: 6 packets included. Each packet makes one gallon of supplemented drinking water. Aids in hydration and nutricion, suited for all ages and all breeds.
  • SAV-A-CHICK POULTRY VITAMINS & ELECTROLYTES: 9 packets included. Each packet makes one gallon of supplemented drinking water. Supports hydration and bird health during hot weather and other stress.
  • DURVET HEALTHY FLOCK PROBIOTICS: Add one scoop per gallon of drinking water. Contains a source of live (viable), naturally occurring microorganisms, plus essential vitamins and electrolytes.

Vitamin K for chickens

Vitamin K is a group of 3 chemicals essential for blood clotting, biosynthesis of proteins, bone composition, and embryo development in chickens and poultry. Adequate intake improves laying performance and health. Deficiencies can result in internal bleeding and blood spots in the eggs.

Vitamin K is one of the four fat-soluble vitamins (the others are vitamins A, D, and E) that dissolve in body fats and oils.

Blood Clotting

The prime function of vitamin K is to regulate bioprocesses for blood clotting. It is vital in regulating multiple blood clotting factors, such as prothrombin, protein C, and other coagulation factors. Continuous intake for chickens is essential to activate clotting in various circumstances, such as:

Bone Health

Vitamin K plays a role in producing principal proteins that regulate bone metabolism. One example is osteocalcin, which plays a crucial role in calcium binding in the core of the chicken bone, the uterus, and the eggshell. High levels of osteocalcin increase bone density and reduce the risk of osteoporosis.

When osteocalcin is not bound to calcium, it acts as a hormone. Birds under stress release osteocalcin from their bones in a fight-or-flight response.

Types of Vitamin K for Chickens

Vitamin k refers to all organic quinone compounds with blood clotting effects.

There are three types of Vitamin K:

  • Vitamin K1, or phylloquinone, is vitamin K derived from plants, especially leafy green vegetables such as collard greens, spinach, broccoli, cabbage, kale, lettuce
  • Vitamin K2, or menaquinone, is vitamin K derived from bacterial fermentation. It can be found in milk, yogurt, cheese, or animal-based feed like eggs, chicken, beef, pork, and fish.
  • Vitamin K3, or menadione, is chemically synthesized vitamin K and is used in the industry as it’s water soluble and has a high stability.

A chicken produces its own vitamin K2 from bacteria in the intestines. However, most of it is made in the last parts of the guts and can’t be absorbed by the body. Instead, it ends up in their droppings, which contain relatively high levels of vitamin K. It’s actually so much that it becomes an important source of vitamin K for chickens eating their droppings in the chicken run.

Visual representation of vitamin k1, k2 and k3. Vitamin k1 and k2 have carbon tails with various double bonds whereas vitamin k3 has a longer tail.

Our birds do not produce enough microbial vitamin K in their guts and need supplemental feeding. This is especially true when they are on antibiotics such as sulphaquinoxaline, which kill the intestinal flora that produces vitamin K2.

Recommended Vitamin K Intake for Chickens

Different sources list different recommendations. We aggregated recent recommendations, as poultry has undergone a tremendous evolution in the last 50 years.

Age & TypeVitamin K Recommendation
Starter hatchlings (0-14 days old)3-5 mg/kg
Growers (2-4 weeks old)3-5 mg/kg
Laying Hens2.5-3 mg/kg
Meat chickens (broilers)5-7 mg/kg
Roosters during mating5-7 mg/kg
Vitamin K recommendations derived from aggregated data taken from different sources such as FEDNA, DSM OVN, NRC, Leeson & Summers, and breeding companies

These are the optimal values for health, growth, and egg laying performance. Higher values are only needed in exceptional circumstances, such as disease. Very high doses won’t harm as it’s virtually impossible to overdose on vitamin K.

Lower intake can be suboptimal but does not necessarily mean there is a deficiency. The minimum requirements to prevent symptoms of deficiency published in Nutrient Requirements of Poultry (National Research Council) are as low as 0.5 to 0.7mg/kg of vitamin K for chickens.

Some breeding companies publish their own guidelines, which are in line with the general recommendations above:

BreedStarter/GrowerRearing PeriodLaying Period
ISA Brown3 mg/kg3 mg/kg3 mg/kg
Hy-Line3.5 mg/kg3.5 mg/kg2.5 mg/kg
Lohmann3 mg/kg3 mg/kg3 mg/kg

Vitamin K in Chicken Feed

Vitamins are organic compounds that can deteriorate and lose their vitamin activity. Although a lot of feed contains vitamin K, the stability varies depending on the type of food. There is a difference between pure commercial feed, vitamin-mineral mixes, or raw materials. Some broiler breeder premixes will only retain 66% of the vitamins after three months of storage.

Vitamin K Stability

Vitamin K is especially sensitive to:

  • heat and higher temperatures, especially with feed storage in summer and hot climates
  • oxidation when the feed comes in contact with oxygen in the air

Raw materials are much more susceptible compared to industrial-manufactured vitamin K. The industrial processes for premixes and laying feed protect the active chemicals during manufacturing and storage. They add antioxidants and micro-coating to prevent oxidation of the fat-soluble vitamin K.

Vitamin K levels in natural food sources vary depending on several factors such as the harvesting conditions, use of fertilizers, genetic modifications, and agricultural practices such as crop rotation.

Vitamin K Interactions

The bioactivity of vitamin K in chickens can be impaired by:

  • excessive levels of vitamin A and E
  • treatment with antibiotics
  • aflatoxins

Vitamin K is a fat-soluble vitamin. When chickens digest vitamin K, the organic compound must compete with other fat-soluble vitamins, like A and E, to compete for the available spots. High levels of vitamins A and E will impair the bioactivity of vitamin K in chickens. Excessive amounts will affect blood clotting, which can suddenly take twice or thrice as long.

Prolonged use of antimicrobials affects the microflora of a chicken and prevents the composition of vitamin K in the guts. The most significant drug is sulfaquinoxaline, an antibiotic used to treat Coccidiosis and Fowl Cholera. When treating chickens with Amprolium and Sulfaquinoxaline, it’s common to supplement with vitamin K.

Aflatoxins are a family of toxins produced by fungi found on common staple foodstuff. The molds grow on hay, corn, wheat, chili peppers, tree nuts, seeds, and almost any crop of food and soil.

Vitamin K for Laying Hens

Research on Leghorns in 2009 shows that higher levels of vitamin K supplementation improve egg laying performance and bone mineralization. Adding vitamin K supplements to a chicken’s diet improves bone structure during growth. It also prevents osteoporosis for laying hens.

Graph showing that vitamin K improves egg production with minimal impact on egg size

The vitamins in a laying hen’s diet directly affect the number of nutrients in the egg. If you want to hatch an egg, vitamin requirements are much higher than for table eggs. Sufficient vitamin levels give the embryo a much higher chance for survival and reinforce the post-hatch growth of chicks.

The vitamin K levels in the egg also vary depending on the diet. Supplementation with vitamin K1 results in eggs high in vitamin K1 and K3 (from the feed). Supplementation with vitamin K3 almost doubles the amount of vitamin K3 in the eggs and has minimal to no vitamin K1 content.

Vitamin K for Meat Chickens

For chickens raised for meat, low levels of vitamin K are associated with the presence of blood and bruises in the carcasses. Bruises and blood spots can occur in all types of muscles.

Blood in chicken meat results from hemorrhages, which is blood loss from damaged blood vessels. They can be caused by extreme environmental conditions, electrical stunning, harsh muscle activity, and everything that can inflict trauma on the muscles. Another problem is the occurrence of petechiae, small round spots on the skin that result from bleeding.

All these symptoms can be linked to capillary fragility caused by marginal deficiencies in vitamin K. With any impaired activity of vitamin K, the blood clotting process takes much longer, ultimately resulting in visual quality defects.

Meat Quality Grading

Vitamin K plays a vital role in preventing blood clots and bruises in poultry meat.

Article §70 in the United States Classes, Standards, and Grades for poultry (AMS70) specifies that bruises and blood clots are quality defects that must be removed from the meat. The Poultry Grading Manual of the United States Department of Agriculture only permits bruises in the flesh if there is no clotting of blood cells. Small clots in the skin can be cut to allow blood extraction with water during the chilling process (leach out).

Immersion chilling involves immersing carcasses in an ice water mix. Evaporate air chilling is a combination of cold air blasts and water misting. Both can extract small blood spots, but the cuts must be considered when determining the meat’s quality. Dark blue or green bruises in the chicken are never allowed and must be removed before grading.

It’s not uncommon in meat chickens to see supplementation with high vitamin A and E levels or antimicrobial supplementation in the chicken’s diet. Both affect the vitamin K levels and have repercussions on the coagulation time, resulting in blood clots and bruises.

Growth and Bone Development

There are several studies that demonstrate the impact of vitamin K on chicken growth:

  • Studies in china on broiler chickens showed that vitamin K improves bone development and feed efficiency. Supplementation of 8 mg/kg improved growth performance during weeks 6 and 7 and bone quality, right after hatching, during weeks 0 to 3. They suggest 8 mg/kg for starters and 2 mg/kg for grower and finisher phases.
  • In turkeys, vitamin K supplementation improves growth for birds with diets low in vitamin D. Vitamin D deficiencies can occur when there is low exposure to sunlight. UV radiation in sunlight is the prime source of vitamin D, as only a few food sources, like fish, contain vitamin D. However, when vitamin D levels are sufficient, there is no effect of extra vitamin K supplementation on the growth of the birds.
  • In Chinese study in 2020 found that vitamin K3 and Bacillus subtilis PB6 supplementation improved broiler growth performance. Supplementation enhanced the structural characteristics of the shin bones, particularly during the growth phase, via osteogenic gene expression and influencing calcium and phosphorus metabolism.

Due to excessive selective breeding for fast growth, some broilers have genetic predispositions for skeletal issues. Vitamin supplementation can partially overcome these issues, and feed additives can alleviate the impact.

However, the influence of vitamin supplementation on skeletal development in practical circumstances is not entirely understood. Research on vitamin K supplementation to prevent leg disorders and bone deformities is inconclusive. Complex interactions between micronutrients complicate the formulation of exact vitamin supplementation quantities.

Vitamin K Deficiency

Chickens with a vitamin K deficiency suffer from chronic reduced vitamin K levels below the absolute minimum to cover their body’s requirements. The chickens’ minimum vitamin K intake is around 0.5 to 0.7mg/kg.

Shortage can be caused either by improper diet or the presence of antagonists like antibiotics or other fat-soluble vitamins, like A and E. For small deficiencies, symptoms only show up after two to three weeks.

Vitamin K level categories: deficiencies, sub-optimal, optimal and high for special cases

Symptoms of vitamin K deficiency

Symptoms of vitamin K deficiency in chickens can be:

When vitamin K deficiency co-occurs with a disease, it can worsen the effects of the disease. For example, in coccidiosis, the intestinal linings of the chicken’s guts can be so damaged it reaches the underlying blood vessels. Vitamin K is essential in blood clotting and restoring the body’s tissue, and deficiencies or sub-optimal vitamin K levels can lead to excessive blood loss.

Vitamin K and Rat Poison

Most commercial rat and rodent poisons are anticoagulants and deplete the rodent’s body from vitamin K. They primarily interfere in the vitamin K cycle by blocking blood clotting factors like prothrombin and proconvertin. Extra toxins in the poison wreak havoc in tiny blood vessels, causing constant internal bleeding.

Eating rat bait can cause vitamin K deficiency and make your chickens prone to internal and external bleeding. It’s a gradual process where the birds ultimately collapse after a week or two. They die from excessive blood loss (hemorrhagic shock) or reduced oxygen flow to the organs (anemia). A terrible and arguably inhumane way for your birds to die.


The antidote is to give the birds very high concentrations of vitamin K1. This limits the amount of internal bleeding caused by the toxins in the poison so that the bird can survive. Active charcoal is only effective in the first hours after ingestion.

Since clinical signs show up after a couple of days, there is time to start treatment if you find out your chickens have been eating poison. Unfortunately, only vitamin K1 can be used as an antidote because it’s absorbed early in the intestines. Vitamin K3 from the drug store is inappropriate for treating rodenticide poisoning in chickens.

Chickens can also get sick when they eat from poisoned rodent carcasses. If you are using rat poison, regularly check the run and coop and remove the rodent carcasses immediately.

Vitamin K Food Sources

Vitamin K1 can be found in dark green leafy vegetables. It plays a role in photosynthesis, where plants use sunlight to synthesize nutrients from water and air. Photosynthesis also relies on chlorophyll, the green pigments of the plant. That’s why dark green leafy vegetables with plenty of green pigments usually contain more vitamin K1.

Vitamin K2 can only be found in animal-sourced foods like cheese and fermented plant foods like soybeans in nattō.

Food SourceMeasureVitamin K1Vitamin K2Safety for chickens
fermented soya
100g1103 μgavoid
raw soybeans are unsafe
Collard Greens
boiled & drained
1/2 cup530 μgsafe
pesticide warning
boiled & drained
1/2 cup445 μgin moderation
high levels of oxalic acid
1/2 cup145 μgin moderation
high levels of oxalic acid
1 cup82 μgsafe
pesticide warning
Egg Yolk1 (18g)< 0.1 μg5.8 μgsafe
100g3-4 μg8-10 μgavoid
contains lactose
whole milk
100g0.16 μg0.9 μgnot safe
contains lactose
100g0.08 μg0.9 μgnot safe
contains lactose
vitamin K levels in common natural food sources and their safety for chickens

Some herbs like oregano and parsley also contain high levels of vitamin K. Although generally safe for chickens, herbs like parsley can be harmful in abundance and disturb eggshell formation. There is a reason why they are toxic to dogs and cats. Even a single herb-packed meal can instantly cause a dog or cat to suffer from digestive issues like diarrhea and vomiting.


When feeding chickens with vegetables, make sure to wash them properly. Vegetables such as spinach, kale, collard, and mustard greens are high in vitamin K but also in the top 3 on the 2022 Dirty Dozen list of the Environmental Working Group (EWG). This list contains all the fruits and vegetables that are cultivated with the most pesticides based on USDA data.

The problem with pesticides is that they can stick, even after the product is washed. The toxins in pesticides are particularly harmful to chickens. If you want to add vitamins to the diet, buy organic vegetables to avoid pesticide consumption in your flock.

When do you need to increase vitamin K levels?

Vitamin K levels in a chicken diet should be increased in case of

  • diseases
  • a combination with antagonists
  • a situation that affects the microflora in the gut

Some diseases can wreak havoc on body tissue and internal linings of organs. Vitamin K is essential for chickens suffering from diseases with internal bleeding and tissue damage like coccidiosis which damages the gut’s intestinal linings. The required vitamin K level for coccidiosis is 8mg/kg, two to four times higher than the recommendation.

Infographic of coccidiosis in chickens where parasites attack the inestinal linings of the chicken's guts where they can cause blood, which can result in bloody droppings

Some antagonists interfere with the activity of vitamin K. High levels of other fat-soluble vitamins like A and E can impair the bioactivity of vitamin K. Anticlotting drugs like the plant-based Dicoumarol can deplete stores of vitamin K.

Any situation affecting a chicken’s microbiome can impact the intestines’ construction and absorption of vitamin K. This can be antimicrobial medication like Sulfaquinoxaline, but also something as simple as heat stress.


Vitamin K is an essential vitamin for blood and bone health in poultry. Chickens synthesize vitamin K2 with bacteria in the guts, but that’s far from sufficient. Vitamin K additives in the feed and supplementation are vital for the metabolism of a chicken.

High levels of vitamin A and E supplementation and antibiotics can interfere with vitamin K production and absorption cycles and cause deficiencies. Chickens with vitamin K deficiencies suffer from internal bleeding, which can have devastating consequences. Accidental ingestion of rat poison can also lead to vitamin K deficiency.

Many dark green leafy vegetables contain high levels of vitamin K1. Check your food’s safety before feeding your chickens with table scraps and leftovers. Vegetables such as spinach, kale, and collard are high in vitamin K, but they also made it to the top 3 in the Dirty Dozen list of pesticides.

Optimal vitamin K levels improve egg-laying performance and bone mineralization in laying hens. For meat chickens, supplementation improves bone development, growth performance, and feed efficiency. It also prevents quality defects with blood and bruises in the meat.

Chickens need higher vitamin K intake when they suffer from diseases, have high vitamin A or E intakes, are treated with antimicrobial medication, or are under any stress.

Related Articles

  • Rooster Booster Poultry Cell: a multi-vitamin, mineral, and amino acid supplement to boost chicken health when under stress; also contains vitamin k
  • Rooster Booster Vitamins & Electrolytes with Lactobacillus: a vitamin and electrolyte supplement that also contains probiotics, does not contain vitamin k
  • Vitamin B12 for Chickens: Vitamin B12 is a water-soluble vitamin among the vitamin B-complexes, which plays a critical role in a chicken’s growth, development, and overall health.
  • Probiotics for Chickens: probiotics are natural supplements with live microorganisms found in the chicken’s digestive system. They promote a healthy gut, boost the immune system, egg laying, and prevent viral and bacterial diseases.
  • Calcium for Chickens: Calcium is essential for chickens as it’s vital for egg production, controls heart rate and blood clotting, promotes a healthy nervous system, supports growth and development, boosts bone strength, activates digestive enzymes, and regulates the body’s pH.
  • Vitamin D for Chickens: Vitamin D is essential for chickens, especially laying hens and chicks. It supports skeleton development and proper immune functioning.
Dr. A. Ibrar, DVM
University of Veterinary and Animal Sciences, IUB, Selçuk Üniversitesi

Dr. Abrir A. is a licensed veterinarian with many years of experience on poultry farms and poultry feed. He has published several scientific articles on poultry diets, amino acids, and prebiotic usage in poultry. Dr. Ibrar is currently pursuing a Ph.D. in animal nutrition and nutritional diseases.


Nick is a chicken fan, writer, and an all-time chicken fanboy holding a master in engineering. He has a passion for chemistry and he loves running with his border collie that's protecting his flock.